AN IMPROVED METHOD OF EXTRACTING GENOMIC DNA FROM PRESERVED TISSUES OF JASMINUM SPP. FOR AFLP AND SSR STUDY
5
Author(s):
CHAYANIKA SARMA , NIRMALA, K.N. , ASHOK, T.H.
Vol - 5, Issue- 6 ,
Page(s) : 194 - 203
(2014 )
DOI : https://doi.org/10.32804/IRJMST
Abstract
In this study, an improved method of genomic DNA extraction from six months old dried leaf tissue of different Jasminum spp in India is described. Two widely used DNA extraction protocols Dellaporta (1983) and Doyle and Doyle (1990) were compared across 56 cultivars and wild types of Jasminum. The modified Doyle and Doyle protocol yielded high quality and quantity of DNA, i.e. average of 1200 to 1500 ng/g of leaf tissue. RNA and protein contamination was minimal with this method. Results from 56 plants were examined. The DNA so extracted was found useful for different marker analysis like AFLP and SSR and consistently amplified all across 56 plants from same and different species of Jasminum. The protocol yielded similar quality and quantity of DNA as extracted from fresh tissues.
- Abdurakhmonov IY, Abdukarimov A (2008) Application of Association Mapping to Understanding the Genetic Diversity of Plant Germplasm Resources. IJPG 8: 1-18.
- Amanlou M, Fazeli MR, Arvin A, Amin HG, Farsam H (2004)Antimicrobial activity of crude methanolic extract of Satureja khuzistanica. Fitoterapia 75:768-770.
- Arias R, Techen N, Rinehart T, Olsen R, Kirkbride JH, ScheffleR BE( 2011) Development of SSR markers for Chionanthus retusus (Oleaceae) and effective discrimination of closely related taxa. HORTSCI 46(1):23-29.
- Bailey LH (1958) Manual of Cultivated Plants. McMillan Co., New York
- Dellaporta SL, Wood J, Ticks J B (1983) A plant molecular DNA minipreparation version 2. Plant Molecular Biology Reporter 1:19-21
- Doyle JJ, Doyle, JL (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemical Bulletin 19:11-15.
- Doyle JJ, Doyle JL (1990) Isolation of plant DNA from fresh tissue. Focus 12:13-15.
- http://www.indiastat.com/agriculture/2/206691/stats.aspx
- Loomis W D (1974) Overcoming problems of phenolics and quinines in the isolation of plant enzymes and organelles. Methods in Enzymology 31:528-544.
- Mukundan S (2000) Characterization of important cultivars of Jasminum species using molecular markers. MSc thesis submitted to College of Agriculture, UAS, Bangalore-65.
- Padmalatha K, Prasad M N V (2006) Optimization of DNA isolation and PCR protocol for RAPD analysis of selected medicinal and aromatic plants of conservation concern from Peninsular India. African Journal of Biotechnology 5: 230-234.
- Porebski S, Bailey LG, Baum BR (1997) Modification of a CTAB DNA extraction protocol for plants containing high polysaccharides and polyphenol component. Plant Molecular Biology Reporter 15, 8-15.
- Ogunkanmi AL, Oboh B, Onifade B, Ogunjobi AA, Taiwo IA, Ogundipe OT (2008) An improved method of extracting genomic DNA from preserved tissues of Capsicum annuum for PCR amplification. EurAsia J Bio Sci 2: 115-119.
- Ribeiro RA, Lovato MB (2007) Comparative analysis of different DNA extraction protocols in fresh and herbarium specimentsof the genus Dalbergia. Genetic Molecular Research 6:173-187.
- Rosa R, Angiolillo A, Guerrero C, Pellegrini M, Rallo L, Besnard G, Bervill A, Martin A (2003) A first linkage map of olive (Olea europaea L.) cultivars using RAPD, AFLP, RFLP and SSR markers. Theor Appl Genet 106:1273–1282.
- Singh AK (2006) Flower Crops. New India publishing Agency, Pitam Pura, New Delhi.
- Veluswamy P, Thangaraj T, Muthuswamy S (1975) A study on germination of seeds of some Jasminum species and clones. S Indian Hort 23:71-72.
- Vos P, Hogers R, Bleeker M, Reijans M, Van de lee T, Hornes M, Fritjers A, Pot J, Peleman J, Kuiper M, Abeau M (1995) AFLP: a new technique for DNA Fingerprinting. Nuc Acids Res, 23:7213–7218.
- Yun-JIang C, Wen-Wu G, Hva-Lin Y, Xlao-Min P, Xivxin D (2003) An Efficient Protocol for Genomic DNA Extraction From Citrus Species. Plant Molecular Biology Reporter 21, 177a- 177g.
- Ziegenhagen B, Schulz F (1993) A procedure for mini-preparation of genomic DNA from needles of silver fir (Abides alba Mill.). Plant Molecular Biology Reporter 11:117-121
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