International Research journal of Management Science and Technology
ISSN 2250 - 1959 (online) ISSN 2348 - 9367 (Print) New DOI : 10.32804/IRJMST
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STUDY OF DEGRADATION PRODUCTS OF CELLULOLYTIC ENZYMES ACTIVITY IN PRESENCE AND ABSENCE OF FUNGICIDES
2 Author(s): DR. ARTI SAXENA,DR. ANURAG SAXENA
Vol - 1, Issue- 1 , Page(s) : 191 - 193 (2009 ) DOI : https://doi.org/10.32804/IRJMST
The inhibitory effects of two fungicides, m- dinitrobenzene and pentachloro phenol were studied on purified carboxymethyl cellulase enzymes, isolated from the fungus Aspergillus niger. The biodegradation products were identified by thin - layer chromatography (TLC), using the solvent system as Acetone : Water : Chloroform : Methanol (8 : 0.5 : 1 : 1, vvvv). Sugars were identified by spraying p - anisidine hydrochloric acid sol. or 20% sulfuric acid in methanol on three ascents developed. It was found that the degradation products of enzymatic hydrolysis of substrate are glucose n cellobiose, as indicated by the Rf values.