International Research journal of Management Science and Technology
ISSN 2250 - 1959 (online) ISSN 2348 - 9367 (Print) New DOI : 10.32804/IRJMST
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INHIBITION STUDIES ON CELLULASE ENZYMES ACTIVITY OF THE FUNGUS NEUROSPORA CRASSA BY USING SUITABLE INHIBITORS
1 Author(s): DR. ARTI SAXENA
Vol - 1, Issue- 1 , Page(s) : 183 - 187 (2009 ) DOI : https://doi.org/10.32804/IRJMST
Cultures of the fungus Neurospora crassa were used for cellulase enzymes production on carboxymethyl cellulose as substrate. The optimum parameters for cellulase production and characterization from the fungus was – Incubation period - 4 days Optimum temperature - 31°C pH optima - 5.0 Optimum substrate - 100 mg of filter paper. The study of cellulase inhibition was carried out by two potent inhibitors, m - dinitrobenzene (MDB) and pentachloro phenol (PCP). Enzyme inhibition showed reduction in fungal cellulolysis. The reduction in cellulolytic activity was 92.5% by MDB & by 86.8% by PCP.